Label-free and rapid optical imaging, detection and sorting of leukemia cells

Funding: Foundation for Polish Science within the TEAM NET programme (POIR.04.04.00-00-16ED/18)

Project period: 01.10.2019-31.12.2023

In this ambitious consortium project along with 5 top-notch research groups from Poland we aim to develop a state-of-the-art diagnostic device for early-stage leukemia detection and characterization by employing microfluidics coupled with stimulated Raman spectroscopy (SRS).


Leukemias are malignancies originating from hematopoietic or lymphoid precursors caused by genetic lesions leading to biochemical, proteomic and metabolomics abnormalities. Current leukemia diagnostics is based on genetic and phenotypic characterization of these changes. Due to complexity of methodologies used, the diagnostic process is often arbitrary and suffers of poor specificity (difficulties in classifying sub-types) which makes it difficult to establish the best therapeutic approach for the patients.

The RApID project (RAman Imaging for Diagnostics) aims to develop a cutting-edge Stimulated Raman Scattering (SRS) microscopic-microfluidic system for non-invasive imaging of live cells and apply it to rapid leukemia cell imaging, diagnostics and sorting. By integrating the multidisciplinary expertise from a broad range of fields (medicine, biology, (bio)chemistry, physics, engineering), consortium partners will characterize Raman spectra of leukemic cells and link them to clinico-biological features of the disease. These data will be thereafter used to design and construct a completely original, microfluidic diagnostic device utilizing SRS microscopy for label-free, rapid leukemia cell imaging, diagnostics and sorting. The device to be developed will be the first diagnostic tool exploiting this technology providing quantitative data and assisting clinicians in cost-effective early diagnostics and follow up of leukemia patients.


  • Ladislav Derzsi, PhD
  • Yurii Promovych, PhD
  • Abhay Kotnala, PhD
  • Shreyas Vasantham, MSc
  • Paweł Jankowski, PhD
  • Karol Makuch, PhD

Former team members

  • prof. Piotr Garstecki
  • Witold Postek, PhD
  • Agnieszka Koszewska, MSc
  • Łukasz Kozoń, MSc

Consortium partners

  • University of Warsaw
  • Laser Centre, Institute of Physical Chemistry, Polish Academy of Sciences
  • Jagiellonian University
  • Institute of Hematology and Transfusion Medicine
  • Medical University of Lodz